FastQCFastQC Report
Wed 27 Apr 2016
SRR616871_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616871_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61552679
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1965270.31928260994131547No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1950700.3169155318162512No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1654030.2687177921207946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT583500.025.72703620
GCGATTT608800.024.65050121
CGATTTT610850.024.5751422
ATAGAAT820450.020.5675352
TATAGAA840050.020.134691
GAATTCG913550.018.4938745
TAGAATT923300.018.3570633
AATTCGC954250.017.6459966
AGAATTC962650.017.6020134
GATTTTT872600.017.49996623
ATTCGCG1017700.016.709437
TTCGCGG1096100.015.676438
TCGCGGC1094050.015.6728999
TCGCGAT1064350.015.44646819
CTCGCGA1087750.015.12452118
CGCGGCC1146250.014.99841610
GCTCGCG1128600.014.9299517
CCGCTCG1146850.014.7806515
CGCTCGC1157200.014.6251216
GCCGCTC1193350.014.26504114
Produced by FastQC (version 0.11.5)