FastQCFastQC Report
Wed 27 Apr 2016
SRR616870_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616870_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62084803
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2092150.3369826268112665No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1912160.30799163524767886No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1822240.2935082197168283TruSeq Adapter, Index 2 (100% over 51bp)
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1569010.25272046043216084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG243200.037.6544739
CTCGTAT251200.037.61113742
TCGTATG263200.036.37507243
TCTCGTA259550.036.01947441
CGTATGC267750.035.68130544
CCGATGT270450.034.96607233
ACCGATG290700.034.15571632
CACCGAT292550.033.9012731
ATCTCGT278600.033.63728740
GTCACCG298850.033.41246829
TCACCGA299950.033.0198930
ACACGTC310000.032.71151713
ACGTCTG310200.032.66141515
CGTCTGA319000.031.75335716
CACGTCT320800.031.68039114
GTATGCC303600.031.43830545
CACACGT330200.030.79215812
GCACACG355950.028.6404511
ATGTATC335000.027.51665536
GTATCTC337000.027.49379738
Produced by FastQC (version 0.11.5)