FastQCFastQC Report
Wed 27 Apr 2016
SRR616869_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616869_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57654293
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT8443871.4645691692030636No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT6025561.0451190512387343No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2152970.3734275260300217No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1381210.23956758952884913No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1038030.18004383472363455No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT787990.13667499140090053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4516850.035.000421
GCGTTTT1859300.034.15135645
CGCGATA2059300.033.0306421
TAATACG1947600.032.64501626
GCGATAA2017500.032.4857522
ATAGAAT4926100.032.3919873
CGATAAT2016950.032.38271323
TATAGAA4911150.032.257392
AATACGA1903350.032.24510627
ATACGAC1965600.032.2231228
TACGACT1988200.032.16448629
CGACTCA1953650.031.98939531
ACGACTC2003950.031.82973530
ATAATAC1976500.031.81254425
TCGCGAT4485850.031.71913120
CGCGATT2038000.031.69740521
GAATTCG4936400.031.6351286
TAGAATT5049150.031.5143054
AGAATTC4994450.031.4029335
GACTCAC2004350.031.35532632
Produced by FastQC (version 0.11.5)