FastQCFastQC Report
Wed 27 Apr 2016
SRR616869_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616869_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57654293
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2466600.4278259036148444No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2241700.3888175335009311No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1784710.3095537048732867No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC728990.1264415817222839TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG127600.029.3943639
CTCGTAT126500.028.67225842
TCTCGTA128700.027.8847141
CGTATGC137250.027.09866344
TCGTATG138900.027.05213743
CGCGATT529600.026.52285820
GCGATTT556650.025.23421
CGATTTT563000.025.00533722
ATCTCGT147850.024.88156940
ACACGTC172300.024.34094613
CGTCTGA173800.023.78133616
ACGTCTG176350.023.6160815
CACGTCT180150.023.30527314
CACACGT190450.022.12756512
ATAGAAT724500.021.8172042
TATAGAA757000.020.9806211
GTAATCT181650.020.77172537
GTATGCC182700.020.65293945
GCACACG211800.020.1095111
GAATTCG819800.019.4229975
Produced by FastQC (version 0.11.5)