FastQCFastQC Report
Wed 27 Apr 2016
SRR616868_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616868_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57291886
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT9986711.7431281630351636No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT6855671.1966214552615706No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1195300.20863338309372467No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1157310.2020024266612553No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT758420.13237825684425888No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT626290.1093156542271972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4629700.038.332771
ATAGAAT4996300.035.87463
TATAGAA4978150.035.7319452
GAATTCG5005050.035.026696
GCGTTTT2141150.035.01737645
TAGAATT5109550.034.9778944
CGCGATA2260200.034.91121
AGAATTC5055000.034.8160175
AATTCGC5021300.034.658687
TAATACG2131050.034.4397926
GCGATAA2203450.034.3332822
ATTCGCG5076950.034.281468
CGATAAT2202350.034.2302823
TCGCGAT4709600.034.16220520
TCGCGGC5045650.033.97321310
CGCGATT2234600.033.9161121
ATACGAC2166750.033.91002728
AATACGA2096750.033.89832727
TACGACT2201200.033.78808229
CTCGCGA4772950.033.76299719
Produced by FastQC (version 0.11.5)