FastQCFastQC Report
Wed 27 Apr 2016
SRR616868_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616868_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57291886
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1551720.27084463583551777No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1345320.23481859193813237No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1317730.23000290128343828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT471950.024.64255120
ATAGAAT543400.023.8833242
GCGATTT493950.023.53588721
CGATTTT503050.023.12354922
TATAGAA573250.022.7618391
GAATTCG620350.021.0833025
TAGAATT632550.020.719353
TATCTCG57450.020.36532839
AGAATTC660350.019.8334624
AATTCGC660350.019.7823546
CGCCAAT63150.019.4177233
GTCACGC66750.018.80866829
ATTCGCG716250.018.3860727
CTCGTAT69600.017.19841842
TCGCGGC787500.016.9654249
TTCGCGG794150.016.8601918
TCGCGAT792150.016.17847819
TCGTATG74400.016.058643
CTCGCGA800250.016.02034418
ACACGTC80750.015.93786313
Produced by FastQC (version 0.11.5)