FastQCFastQC Report
Wed 27 Apr 2016
SRR616867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47940292
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT7956291.6596248516800856No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT5345631.11505995833317No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT910620.18994878045381952No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840600.17534311221967527No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT659500.13756695516164147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA3586450.038.504011
ATAGAAT3903700.035.718383
TATAGAA3896050.035.5372922
GAATTCG3916400.034.881526
TAGAATT3992300.034.8450974
GCGTTTT1712250.034.7310745
CGCGATA1810750.034.66951821
AGAATTC3961050.034.615965
AATTCGC3929600.034.522527
TAATACG1709450.034.25288426
ATTCGCG3967650.034.159878
GCGATAA1776750.034.03976422
TCGCGAT3704250.033.91507720
CGATAAT1779950.033.88113423
ATACGAC1729500.033.818728
AATACGA1677850.033.76695327
TCGCGGC3953900.033.75453610
TACGACT1758500.033.62162429
TTCGCGG4013800.033.557759
CGCGATT1742350.033.52613421
Produced by FastQC (version 0.11.5)