FastQCFastQC Report
Wed 27 Apr 2016
SRR616867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47940292
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1284290.267893654047831No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1144300.23869274721981254No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT868870.1812400308283479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT408200.024.62168120
ATAGAAT458250.024.5113012
GCGATTT430400.023.32033321
TATAGAA489100.023.1431031
CGATTTT435500.023.08856622
GAATTCG521450.021.7164615
TAGAATT531900.021.2559663
AATTCGC550100.020.5363546
AGAATTC556250.020.4104234
ATTCGCG600350.018.9785927
TCGCGGC663600.017.3086879
TTCGCGG667000.017.2676838
TCGCGAT670200.016.44666319
CTCGCGA678450.016.24003818
GATTTTT630850.016.1921523
CGCGGCC714450.016.15243110
GCTCGCG713400.015.92381317
CCGCTCG717500.015.88934615
CGCTCGC726350.015.68955216
GGCCGCT759700.015.071877513
Produced by FastQC (version 0.11.5)