FastQCFastQC Report
Wed 27 Apr 2016
SRR616866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48421523
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT8765301.8102074154090528No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT6246111.2899449693063145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1542420.3185401665288388No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1086740.22443325460869953No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT670340.13843843779965367No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT631100.13033460347787904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4097850.037.8035771
ATAGAAT4415450.035.4728433
TATAGAA4397300.035.3061072
GCGTTTT1859550.035.29472745
CGCGATA1979750.034.91167421
GAATTCG4409450.034.78966
TAGAATT4511950.034.6076164
AGAATTC4463850.034.5081445
TAATACG1871000.034.47946526
AATTCGC4419850.034.475317
GCGATAA1940350.034.36448722
CGATAAT1942350.034.17896323
AATACGA1831650.034.11883527
ATTCGCG4461900.034.1116528
ATACGAC1898300.034.0740528
TCGCGAT4130900.034.04385820
TACGACT1926400.033.96464529
TCGCGGC4430350.033.8263110
CGCGATT1942250.033.8198921
CGACTCA1898650.033.77495631
Produced by FastQC (version 0.11.5)