FastQCFastQC Report
Wed 27 Apr 2016
SRR616866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48421523
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2054760.42434848651910434No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1748370.3610729055341774No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1120080.23131862250594637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG74250.030.33311739
CTCGTAT87950.026.86216242
TCTCGTA86750.026.71472541
ACACGTC103450.024.7942313
TCGTATG97100.024.63210343
CGCGATT397750.024.13722820
CGTATGC99500.024.06057744
CACCGAT105850.023.74309231
GTCACCG107150.023.66500929
CCGATGT103450.023.1194633
ACCGATG109400.023.09603332
GCGATTT420400.022.8956521
ACGTCTG112750.022.7890315
ATAGAAT488900.022.4820582
CGATTTT428650.022.4549922
ATCTCGT105100.022.2858540
TCACCGA114700.021.9895930
CACGTCT118550.021.73102414
TATAGAA520500.021.19321
CGTCTGA121700.021.15006416
Produced by FastQC (version 0.11.5)