FastQCFastQC Report
Wed 27 Apr 2016
SRR616865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48795992
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT7373891.5111671466787682No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT7054661.4457457899411081No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5623831.1525188380225984No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1759000.36048042634321276No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT638200.130789430410596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4231600.039.291561
CGCGATA1941050.034.86401421
ATAGAAT4774300.034.7953073
TATAGAA4743350.034.7851372
TAATACG1826950.034.3978326
GCGATAA1894300.034.3626122
ATACGAC1846750.033.9610728
AATACGA1786800.033.9335627
TAGAATT4864250.033.92614
CGATAAT1900150.033.82241423
TACGACT1867500.033.816729
GAATTCG4840100.033.610356
GCGTTTT1785500.033.52223645
AGAATTC4859350.033.5207025
ATAATAC1870650.033.3544425
AATTCGC4869650.033.3522267
CGACTCA1861700.033.21004531
ACGACTC1900900.033.16065230
ATTCGCG4890250.032.9272238
GATAATA1914750.032.8499424
Produced by FastQC (version 0.11.5)