FastQCFastQC Report
Wed 27 Apr 2016
SRR616865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48795992
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7672541.5723709439086717No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5655991.1591095432592087No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT3149490.6454403058349547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT729300.030.57191320
GCGATTT754100.029.56053421
CGATTTT759500.029.40071922
ATAGAAT848750.029.0567742
TATAGAA857550.028.7511941
TAGAATT924100.026.9637853
GAATTCG932800.026.7750115
AGAATTC950100.026.0909294
AATTCGC964200.025.7746726
ATTCGCG1023800.024.5115457
GATTTTT963650.023.32857923
TCGCGGC1085800.023.3212229
TTCGCGG1096500.023.1921128
TCGCGAT1051850.022.41623519
CCGCTCG1133250.022.26341615
GCTCGCG1129250.022.2166717
CGCGGCC1139950.022.19769310
CTCGCGA1067400.022.16344618
CGCTCGC1145650.021.9595816
CGCGGTT48750.021.5986420
Produced by FastQC (version 0.11.5)