FastQCFastQC Report
Wed 27 Apr 2016
SRR616864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49143509
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT9121631.8561210189528794No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT8430381.7154615475260426No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7300611.4855695387970769No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1389440.28273113342394823No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1156360.23530269277271185No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT775040.15770953596333548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4980100.039.2306371
ATAGAAT5436950.035.968513
TATAGAA5418150.035.82692
TAGAATT5547150.035.0825734
GAATTCG5508500.034.7252926
AGAATTC5539750.034.6609655
AATTCGC5540200.034.4674847
GCGTTTT2132450.034.43917545
CGCGATA2423450.034.4339821
ATTCGCG5570900.034.027818
TAATACG2263250.033.999926
GCGATAA2369850.033.8332622
TCGCGGC5522950.033.78631610
AATACGA2199350.033.7347227
TTCGCGG5643700.033.6996429
ATACGAC2278400.033.58811628
TCGCGAT5081500.033.5333520
TACGACT2300400.033.45110329
CGCGGCC5609700.033.2592611
CGATAAT2384450.033.2476423
Produced by FastQC (version 0.11.5)