FastQCFastQC Report
Wed 27 Apr 2016
SRR616864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49143509
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8870151.804948441919359No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8863741.803644098755748No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2542900.5174437177451044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT700000.028.4844362
CGCGATT634150.028.39332220
TATAGAA726200.027.4856721
CGATTTT656850.027.38467622
GCGATTT657550.027.36921321
TATCTCG53800.026.26259239
GAATTCG777350.025.9065935
TAGAATT787800.025.5372473
AGAATTC791000.025.3429154
AATTCGC813500.024.6779276
CGCCAAT63150.023.94149633
ATTCGCG869750.023.3509397
GTCACGC67100.022.46506329
CTCGTAT63400.022.28635242
TTCGCGG940650.021.9592388
TCGCGGC942100.021.8227529
TCTCGTA66100.020.7633341
GATTTTT876900.020.75648523
CGCGGCC994900.020.71439710
TCGCGAT928650.020.69245519
Produced by FastQC (version 0.11.5)