FastQCFastQC Report
Wed 27 Apr 2016
SRR616863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53767652
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT10484741.950008901262789No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT8467151.5747665529452541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6256791.1636717928467473No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1302750.24229252190517822No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1246880.23190151580359136No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT766270.1425150571946121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA5184850.039.8818441
ATAGAAT5667900.036.462833
TATAGAA5652500.036.330012
TAGAATT5784000.035.5024154
GAATTCG5755750.035.136666
AGAATTC5784900.035.045985
AATTCGC5797950.034.8316087
GCGTTTT2487600.034.38700545
ATTCGCG5841750.034.3366628
CGCGATA2649400.034.3168121
TCGCGGC5800150.034.0680910
TTCGCGG5922000.033.9977049
TAATACG2500300.033.97157726
GCGATAA2589200.033.8538122
AATACGA2426400.033.66148427
ATACGAC2515800.033.62711728
TCGCGAT5439150.033.529220
CGCGGCC5900250.033.5167411
TACGACT2547650.033.39692729
CGATAAT2615550.033.29741323
Produced by FastQC (version 0.11.5)