FastQCFastQC Report
Wed 27 Apr 2016
SRR616863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53767652
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8656551.6099921938194361No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760581.2573693937760198No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2342950.4357545685647571No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT694050.028.4563312
CGCGATT642100.027.21133420
TATAGAA734650.026.9646111
GCGATTT665450.026.21932621
CGATTTT668900.026.12781722
GAATTCG775050.025.6786585
TAGAATT789000.025.2702673
AGAATTC804000.024.6840764
AATTCGC828150.023.9832736
ATTCGCG903350.022.2383167
TCGCGGC973300.020.8088179
TTCGCGG978600.020.765098
CGCGGCC1024550.019.80530510
TCGCGAT983550.019.3910319
GCTCGCG1038050.019.36128617
GATTTTT916250.019.30273823
CCGCTCG1047900.019.29743415
CTCGCGA991000.019.2929318
CGCTCGC1057550.019.06816116
GCCGCTC1082100.018.69169214
Produced by FastQC (version 0.11.5)