FastQCFastQC Report
Wed 27 Apr 2016
SRR616862_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616862_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43759296
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT7939171.8142819299469533No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT5768601.3182570396013682No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2058120.47032749338563395No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT893060.2040846360965222No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT731470.16715762520493932No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT545620.1246866494378703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA3953050.039.077251
ATAGAAT4262200.036.23333
TATAGAA4253250.036.069182
TAGAATT4339550.035.368114
GAATTCG4300450.035.1745536
CGCGATA1940200.034.98834621
AATTCGC4319600.034.96357
AGAATTC4350150.034.8433885
GCGTTTT1841050.034.7476545
ATTCGCG4344550.034.4979678
TAATACG1833350.034.46576326
GCGATAA1905700.034.34237322
TCGCGGC4306350.034.27237310
AATACGA1785300.034.1686627
TTCGCGG4407350.034.143939
ATACGAC1854650.034.08144828
TCGCGAT3980700.034.07673320
TACGACT1881000.033.8399929
CGCGATT1841500.033.7490121
CGCGGCC4381350.033.73328411
Produced by FastQC (version 0.11.5)