FastQCFastQC Report
Wed 27 Apr 2016
SRR616862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43759296
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2581000.5898175327135061No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2535480.5794151715786287No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1367850.31258501050839577No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC525950.12019160454500913TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG85650.032.415139
CTCGTAT90900.030.59306742
TCTCGTA91850.029.98266441
GTCACCG109000.028.64959729
ACCGATG111900.027.76637332
CCGATGT106350.027.69221533
ACACGTC118050.027.36815313
CACCGAT115150.027.09992431
CGCGATT441850.026.35063420
TCACCGA117400.026.33141330
ACGTCTG123800.026.04249615
TCGTATG109350.025.98684143
CGTCTGA126850.025.38082116
CGATTTT461300.025.23472222
GCGATTT462250.025.22666521
ATCTCGT110650.025.07111540
CACGTCT129450.024.81893714
ATAGAAT548750.023.9284842
CGTATGC119650.023.8895544
CACACGT136850.023.65772812
Produced by FastQC (version 0.11.5)