FastQCFastQC Report
Wed 27 Apr 2016
SRR616861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48568051
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8878441.8280412364086835No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8676841.7865324676092107No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT3097650.63779582178416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT792950.029.4130632
TATAGAA821800.028.4800871
CGCGATT760650.028.16585520
CGATTTT783900.027.35056722
GCGATTT786200.027.27627621
TAGAATT877550.026.7310833
GAATTCG890500.026.4080415
AGAATTC895600.026.1822954
AATTCGC942850.024.8820236
ATTCGCG1026450.023.0330377
GATTTTT999850.021.61905123
TCGCGGC1104950.021.6186289
TTCGCGG1108200.021.6039548
TCGCGAT1099150.020.78508619
CGCGGCC1155500.020.69428810
CTCGCGA1112050.020.56610118
GCTCGCG1149400.020.54377217
CCGCTCG1156350.020.5273115
CTCGTAT59750.020.447542
CGCTCGC1167500.020.29465316
Produced by FastQC (version 0.11.5)