FastQCFastQC Report
Wed 27 Apr 2016
SRR616860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56278076
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4634750.8235445007039686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4252530.7556281774806942No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT2406550.4276176747762308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT694750.027.36591120
CGATTTT719600.026.43651622
GCGATTT723300.026.2981721
ATAGAAT882350.023.8438932
TATCTCG60750.023.5553939
TATAGAA917450.023.0201321
GAATTCG975950.021.7305955
TAGAATT978700.021.5982693
CGCCAAT72200.021.12897933
AGAATTC1001250.021.1140824
AATTCGC1015350.020.8030916
GATTTTT966700.019.92586723
CTCGTAT71950.019.85732842
ATTCGCG1093100.019.5127747
TCTCGTA72750.019.32970841
GTCACGC78650.019.0245329
TCGCGGC1169450.018.4600949
TTCGCGG1178100.018.3684798
TCGCGAT1144100.017.92911719
GCTCGCG1206950.017.64792417
Produced by FastQC (version 0.11.5)