FastQCFastQC Report
Wed 27 Apr 2016
SRR616859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51370397
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6316531.2296050583373923No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5146081.0017598267733847No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT3320540.6463917341343498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT845900.029.25607720
GCGATTT870900.028.41883721
CGATTTT874100.028.35340922
ATAGAAT1009450.026.7022362
TATAGAA1031350.026.2107771
AGAATTC1098650.024.6365174
GAATTCG1106350.024.593175
TAGAATT1109900.024.4090963
AATTCGC1155850.023.458066
GATTTTT1099350.022.73444223
ATTCGCG1240550.022.0813277
TCGCGGC1310950.021.0928969
TTCGCGG1323700.020.9594158
TCGCGAT1290300.020.37212219
CGCGGCC1360900.020.34681710
CCGCTCG1358900.020.199615
GCTCGCG1354000.020.13145617
CTCGCGA1311450.020.0812218
CGCTCGC1372400.019.94024516
GCCGCTC1390900.019.7850214
Produced by FastQC (version 0.11.5)