FastQCFastQC Report
Wed 27 Apr 2016
SRR616858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52714279
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT13510302.5629298657390347No Hit
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT13381392.5384753910795212No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13198782.503833923252559No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT3127170.5932301568612938No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1318160.250057484424666No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT927850.176014927568297No Hit
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGTGTTTT872930.16559649805700652No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTC640510.12150597753599172No Hit
GTATAGAATTCGCGGCCGCTCGCGATGTATAGAATTCGCGGCCGCTCGCGA629560.11942874149905379No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550370.1044062463606872No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTA549020.10415014876709212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA6812650.038.5187231
TCTCGCG69100.035.62652218
CGCGATA3194400.035.42424421
GCGTTTT2621000.035.41680545
GCGATAA3092600.035.04469722
TAATACG2898500.035.01049826
CGATAAT3031750.034.8977723
ATAGAAT7552200.034.7917253
TATAGAA7525200.034.7404172
AATACGA2826500.034.7239727
ATACGAC2919950.034.60425628
TACGACT2957050.034.57792729
ATAATAC2931150.034.56738325
CGACTCA2921100.034.4026931
ACGACTC2971800.034.37450830
TAGAATT7693200.034.1457864
AGAATTC7621050.034.1137965
GAATTCG7610050.034.006526
GACTCAC2959250.034.00251832
GATAATA3022200.033.98895324
Produced by FastQC (version 0.11.5)