FastQCFastQC Report
Wed 27 Apr 2016
SRR616858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52714279
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13436922.5490095387627325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12783932.425136081250395No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT4852040.9204413096497062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT1134000.030.7208942
CGCGATT1054450.030.56041320
TATAGAA1158000.030.179051
CGATTTT1080000.029.88743222
GCGATTT1080700.029.8035321
TAGAATT1240400.028.216753
AGAATTC1244150.028.0575564
GAATTCG1255700.027.9661165
AATTCGC1312600.026.6508376
ATTCGCG1415000.025.0322447
GATTTTT1298200.024.97685423
TATCTCG56800.024.4803939
TCGCGGC1490750.024.0440129
TTCGCGG1506450.023.8770688
CGCGGCC1540850.023.25639310
TCGCGAT1476150.022.9986319
GCTCGCG1544700.022.84885817
CCGCTCG1555100.022.83205215
CTCGCGA1493300.022.76747718
CGCTCGC1567300.022.58254816
Produced by FastQC (version 0.11.5)