FastQCFastQC Report
Wed 27 Apr 2016
SRR616857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57170532
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT701354112.267755353404793No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA3464550.6060027568048518No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG3415390.5974039213068718No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3075520.5379554627898162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC1034710.18098659638150646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG730690.12780885089542282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAAACTCATGC652020.11404826528463999No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGAGAGATGGCTCA641130.11214343781163344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15325250.092.91078
GTGGTAT15335200.092.860176
GTATCAA15350750.092.7068949
AGTGGTA15318750.092.650255
AAGCAGT15324550.092.620881
TGGTATC15430100.092.254047
AGCAGTG15510100.091.5636752
GCAGTGG15588400.091.2301943
CAGTGGT15690850.090.488414
CAACGCA15311900.046.79161512-13
TCAACGC15278800.046.68687412-13
ACGCAGA15355550.046.6711814-15
ATCAACG15342900.046.65487710-11
AACGCAG15353550.046.46365714-15
AGAGTAC15158600.046.3689818-19
TATCAAC15371450.046.3652110-11
CGCAGAG15481350.046.06213816-17
GCAGAGT15458200.046.0519716-17
CAGAGTA15302150.045.94359618-19
GAGTACT8270750.045.8998120-21
Produced by FastQC (version 0.11.5)