FastQCFastQC Report
Wed 27 Apr 2016
SRR616857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57170532
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19782993.4603473691656395No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4408440.7711035468412293No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3056070.5345533604619946No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA703560.12306339916515031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA663550.055.002388
GTGGTAT684700.053.1091546
GTATCAA700900.052.159479
AGTGGTA702000.050.8909725
AAGCAGT758750.046.2078631
TGGTATC795350.045.8220677
CAGTGGT875750.040.9514544
AGCAGTG908800.039.3473932
GCAGTGG948650.037.9747663
CAACGCA630650.034.42333212-13
ATCAACG672950.032.48497810-11
ACGCAGA679450.031.89511314-15
TCAACGC610450.030.06523712-13
AGAGTAC750400.028.34721218-19
AACGCAG672250.027.23781814-15
GAGTACT648250.025.60055720-21
TATCAAC726400.025.34751910-11
AGTACTT778650.024.3205320-21
GCAGAGT889650.024.15628616-17
CGCAGAG821550.022.36016716-17
Produced by FastQC (version 0.11.5)