FastQCFastQC Report
Wed 27 Apr 2016
SRR616856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64483644
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT907317314.070502901479948No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG4714460.7311094267563415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA4492010.6966123068355132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3757490.5827043521299758No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC1445650.22418863301211697No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGAGAGATGGCTCA987450.15313185464518722No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG949150.14719236400473895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAAACTCATGC782550.12135635510921187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT20289850.093.041776
GGTATCA20291550.093.014358
AAGCAGT20238500.092.95051
GTATCAA20315800.092.864179
AGTGGTA20263250.092.827995
TGGTATC20377500.092.601567
AGCAGTG20452600.092.012992
GCAGTGG20552000.091.6924063
CAGTGGT20743850.090.695424
CAACGCA20280650.046.81785612-13
TCAACGC20234950.046.73772412-13
ACGCAGA20332600.046.71379514-15
ATCAACG20319650.046.69288610-11
AACGCAG20318450.046.55643514-15
AGAGTAC20013700.046.51229518-19
TATCAAC20327000.046.4780310-11
GCAGAGT20397200.046.2301416-17
CGCAGAG20458300.046.2200416-17
CAGAGTA20175350.046.18957518-19
GAGTACT10692100.046.18688620-21
Produced by FastQC (version 0.11.5)