FastQCFastQC Report
Wed 27 Apr 2016
SRR616856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64483644
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25047183.884268699206887No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5079420.7877067245145142No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4140340.6420759968217676No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA893110.13850178814336236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA853100.058.509868
GTGGTAT859200.057.774246
GTATCAA893750.055.7742849
AGTGGTA881700.055.4013985
TGGTATC957800.051.935457
AAGCAGT946900.050.72891
CAGTGGT1093050.044.7760164
AGCAGTG1112600.043.853012
GCAGTGG1144750.042.9405633
CAACGCA820900.034.94784512-13
ATCAACG857250.033.5764510-11
ACGCAGA863050.033.18050814-15
TCAACGC787100.031.80199412-13
AGAGTAC916650.030.50162118-19
AACGCAG852500.029.2647914-15
GAGTACT785150.028.87403120-21
TATCAAC916550.027.33865410-11
AGTACTT931250.027.03200320-21
GCAGAGT1086600.026.03290216-17
CGCAGAG1017050.024.53234916-17
Produced by FastQC (version 0.11.5)