FastQCFastQC Report
Wed 27 Apr 2016
SRR616855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63080464
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT705087611.177590577012877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA4231410.670795636506415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG4113820.6521543658905236No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3777970.5989128424927248No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC1265630.20063739543830875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG900810.1428033249723718No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGAGAGATGGCTCA847720.1343870901139852No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAAACTCATGC677640.10742470125140488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16091250.092.800876
GGTATCA16088300.092.781958
GTATCAA16114750.092.592159
AGTGGTA16092600.092.457625
AAGCAGT16092900.092.4543151
TGGTATC16207600.092.101557
AGCAGTG16291100.091.385592
GCAGTGG16375450.091.032033
CAGTGGT16540100.089.991844
CAACGCA16083800.046.71702612-13
TCAACGC16035550.046.6331112-13
ACGCAGA16129900.046.60156614-15
ATCAACG16111500.046.590310-11
AACGCAG16119300.046.3955114-15
TATCAAC16131650.046.3063210-11
AGAGTAC15905250.046.30151718-19
CGCAGAG16249800.046.01930216-17
GCAGAGT16236550.045.98938416-17
GAGTACT8367100.045.85477420-21
CAGAGTA16084400.045.82959718-19
Produced by FastQC (version 0.11.5)