FastQCFastQC Report
Wed 27 Apr 2016
SRR616855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63080464
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19519323.0943526350725636No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4382980.6948236778981207No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4126600.654180349719685No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA677490.10740092209848044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA841200.061.799498
GTGGTAT860150.060.018416
GTATCAA890100.058.377699
AGTGGTA884250.057.6629375
TGGTATC972950.053.235777
AAGCAGT963700.051.9158861
CAGTGGT1086050.047.0185554
AGCAGTG1145250.044.3976942
GCAGTGG1166900.043.9318053
CAACGCA813550.036.13664212-13
ATCAACG852400.034.6536510-11
ACGCAGA863700.034.03846714-15
TCAACGC779450.033.34222812-13
AGAGTAC931400.031.02588318-19
AACGCAG857100.030.23571214-15
GAGTACT814200.029.38696720-21
TATCAAC907350.028.73099310-11
AGTACTT967600.027.15791120-21
GCAGAGT1081400.026.97746716-17
CGCAGAG1000200.025.91224316-17
Produced by FastQC (version 0.11.5)