FastQCFastQC Report
Wed 27 Apr 2016
SRR616854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62143803
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59497469.574158182755568No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA3499620.5631486698681766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG3315770.5335640626950365No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3247540.5225846895787823No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGGCTGGTGAGATGGCTC1132700.18227078893127927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGAGAGATGGCTCAG736860.11857336764536282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGAGAGATGGCTCA720860.11599869418999027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13451000.092.49288
GTGGTAT13461300.092.451776
GTATCAA13465750.092.344939
AAGCAGT13470450.092.1081
AGTGGTA13473100.092.0936365
TGGTATC13574550.091.65667
AGCAGTG13694350.090.65092
GCAGTGG13737400.090.495553
CAGTGGT13860400.089.54834
CAACGCA13419000.046.7099912-13
ACGCAGA13467050.046.57098414-15
TCAACGC13382750.046.56168412-13
ATCAACG13457100.046.524610-11
AACGCAG13460200.046.29694714-15
AGAGTAC13297500.046.24794818-19
TATCAAC13491700.046.14446310-11
CGCAGAG13607400.045.8040216-17
GCAGAGT13617400.045.7790916-17
GAGTACT7090050.045.7466720-21
CAGAGTA13453750.045.67346618-19
Produced by FastQC (version 0.11.5)