FastQCFastQC Report
Wed 27 Apr 2016
SRR616854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62143803
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16627902.675713296786809No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3875440.6236245309930581No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2603160.4188929345054727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA799700.058.3543438
GTGGTAT808150.057.4212656
GTATCAA819100.056.925869
AGTGGTA834200.055.0026935
AAGCAGT893650.050.464341
TGGTATC937550.049.6375967
CAGTGGT1037150.044.4687774
GCAGTGG1095150.042.1570553
AGCAGTG1105650.041.4947972
CAACGCA740650.036.19834512-13
ATCAACG789450.034.07162510-11
ACGCAGA796100.033.74866514-15
TCAACGC716400.032.5438912-13
AGAGTAC843200.031.36170418-19
AACGCAG791750.029.40774214-15
GAGTACT727450.029.31981320-21
TATCAAC847800.027.59763310-11
AGTACTT878100.027.10504220-21
GCAGAGT1012050.026.34796516-17
CGCAGAG936850.024.91136616-17
Produced by FastQC (version 0.11.5)