FastQCFastQC Report
Wed 27 Apr 2016
SRR616853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60606594
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT14646772.4166957806604343No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT10700051.7654927118986425No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT934130.1541300935010471No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTC710720.11726776792637449No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTA692240.1142185947621475No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTG607910.10030426722214417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA5156050.084.0993961
ATAGAAT5530650.078.4140553
GAATTCG5510800.078.048746
TATAGAA5575850.077.707632
AATTCGC5556550.077.1721047
ATTCGCG5640250.076.240848
TAGAATT5721400.075.814614
AGAATTC5737050.075.335455
TTCGCGG5730500.074.991499
CGCGATT2753650.040.918720-21
CGCGATA2615950.040.00272420-21
CTCGCGA5626600.039.80624818-19
CGATTTT2767000.039.5235222-23
TAATACG2479450.039.47928626-27
GCGATAA2553950.039.4422-23
CGCGGCC5801850.039.26737610-11
CGCTCGC5824950.039.17466416-17
CGATAAT2553000.039.07240722-23
ATACGAC2518950.039.0715428-29
TACGACT2588400.038.9529328-29
Produced by FastQC (version 0.11.5)