FastQCFastQC Report
Wed 27 Apr 2016
SRR616853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60606594
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT1316250.21717933860464092No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040150.17162323954386877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTCG752400.040.2960555
ATAGAAT734350.040.2694322
TATAGAA783650.037.8481751
AATTCGC806250.037.5381166
ATTCGCG903550.033.710897
TAGAATT921450.033.376643
AGAATTC957900.031.8343474
TCGCGGC1027850.029.7909879
TTCGCGG1046450.029.3067728
CGCGATT629950.021.22229420-21
GCGATTT690500.019.27205520-21
CGATTTT713850.018.81430622-23
CTCGCGA1044100.015.49010618-19
TCGCGAT1029950.015.3001618-19
GCTCGCG1099800.014.61734216-17
CGCTCGC1134950.014.61158816-17
CCGCTCG1117850.014.4683514-15
CGCGGCC1169700.014.14510410-11
GCCGCTC1205850.013.864614514-15
GGCCGCT1195900.013.60528512-13
Produced by FastQC (version 0.11.5)