FastQCFastQC Report
Wed 27 Apr 2016
SRR616852_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616852_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63633623
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT17393812.7334307210513535No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT12678361.9923995212405239No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT856420.13458608195230376No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTC842450.1323907016892626No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTA812080.12761806757411878No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTG720070.11315873056607197No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA6097150.084.8688661
ATAGAAT6463400.080.064863
GAATTCG6460400.079.518696
TATAGAA6507650.079.449732
AATTCGC6507100.078.6929867
ATTCGCG6592950.077.87488
TAGAATT6682050.077.5116654
AGAATTC6689050.077.131065
TTCGCGG6691000.076.692799
CGCGATT3194200.041.27456320-21
GCGATAA2997300.040.441322-23
TAATACG2908750.040.3816326-27
CGCGATA3075450.040.33807420-21
CTCGCGA6557300.040.28444718-19
ATACGAC2944550.040.13485328-29
CGATTTT3202550.039.9803622-23
TCGCGAT6544400.039.9202120-21
TCGCGGC6661350.039.90429310-11
CGCGGCC6760050.039.73558410-11
CGCTCGC6778200.039.66996416-17
Produced by FastQC (version 0.11.5)