FastQCFastQC Report
Wed 27 Apr 2016
SRR616852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63633623
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT1154980.18150467403058287No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1117840.17566813695332104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATTCG711000.038.5958945
ATAGAAT703650.037.9866332
AATTCGC763900.035.808856
TATAGAA765800.035.092821
ATTCGCG857750.032.0787777
TAGAATT874300.031.9620463
AGAATTC920600.029.9938744
TCGCGGC986900.028.0825469
TTCGCGG1003200.027.678248
CGCGATT608900.019.63198920-21
GCGATTT668950.017.74210720-21
CGATTTT698350.017.090222-23
CTCGCGA998150.014.51715118-19
TCGCGAT987000.014.36653818-19
GCTCGCG1062100.013.71905616-17
CGCTCGC1098750.013.62388216-17
CCGCTCG1082500.013.52627914-15
CGCGGCC1148450.013.00125910-11
GCCGCTC1163150.012.89205414-15
CGCGATA383250.012.79077914-15
Produced by FastQC (version 0.11.5)