FastQCFastQC Report
Wed 27 Apr 2016
SRR616851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63177316
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT13477432.133270428898879No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT9731211.540301268892145No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT1083110.1714396983879467No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTC650990.10304173099091453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4722850.084.483351
ATAGAAT5134500.077.7481163
GAATTCG5136150.077.104666
TATAGAA5184800.076.918622
AATTCGC5203450.075.908617
ATTCGCG5298750.074.76818
TAGAATT5352050.074.613464
AGAATTC5360350.074.284315
TTCGCGG5397100.073.322739
CGCGATT2608600.040.42098620-21
CGCGATA2440750.039.26508320-21
CTCGCGA5300600.039.26088718-19
CGATTTT2626950.038.94333622-23
CGCGGCC5453800.038.8380910-11
CGCTCGC5496100.038.58442716-17
CGATAAT2391700.038.19096822-23
GCCGCTC5560200.038.1818114-15
CGACTCA2432850.038.1392330-31
TACGACT2422750.038.12597328-29
GCGATAA2387350.038.0278122-23
Produced by FastQC (version 0.11.5)