FastQCFastQC Report
Wed 27 Apr 2016
SRR616851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63177316
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT1534060.24281816593791353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA867900.1373752566506624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT795050.042.2109762
GAATTCG813450.042.190965
TATAGAA857100.039.2941861
AATTCGC890650.038.4416926
TAGAATT997150.034.803483
ATTCGCG1006500.034.214817
AGAATTC1019800.033.770094
TCGCGGC1132900.030.6232689
TTCGCGG1148950.030.2367958
CGCGATT702450.021.58647220-21
GCGATTT766250.019.83862120-21
CGATTTT787600.019.30082722-23
CTCGCGA1167200.015.51572418-19
TCGCGAT1154600.015.381218-19
GCTCGCG1231100.014.79137616-17
CGCTCGC1259200.014.76814216-17
AGTCGCG31450.014.7626951
CCGCTCG1245600.014.66111614-15
CGCGGCC1271000.014.54515710-11
GCCGCTC1333800.014.04173214-15
Produced by FastQC (version 0.11.5)