FastQCFastQC Report
Wed 27 Apr 2016
SRR616850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63404999
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT16391002.5851273966584243No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT11861741.8707893994288998No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT1145680.180692377268234No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTC788580.12437189692251238No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTA769000.12128381233788836No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTG666910.10518255823961137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA5766950.084.672171
ATAGAAT6242100.078.2303543
TATAGAA6277100.077.728322
GAATTCG6242900.077.600236
AATTCGC6301850.076.636057
TAGAATT6455800.075.662824
ATTCGCG6408500.075.5790258
AGAATTC6443100.075.549585
TTCGCGG6506200.074.380779
CGCGATT3111450.041.01877620-21
CGCGATA2964400.039.80191820-21
CTCGCGA6391050.039.73543518-19
CGATTTT3126750.039.64472622-23
CGCGGCC6569900.039.30679710-11
CGCTCGC6601700.039.14449316-17
TAATACG2804500.038.9515226-27
GCGATAA2895250.038.90996622-23
CGATAAT2896750.038.8553922-23
GCCGCTC6667050.038.81403414-15
TACGACT2920650.038.79883628-29
Produced by FastQC (version 0.11.5)