FastQCFastQC Report
Wed 27 Apr 2016
SRR616850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63404999
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT1744560.27514549759712165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130870.17835659929590095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT857100.043.9632152
GAATTCG887700.043.768145
TATAGAA917050.041.1294481
AATTCGC957050.040.5744176
TAGAATT1078000.036.5433243
ATTCGCG1076900.036.20857
AGAATTC1084400.035.9163934
TCGCGGC1213650.032.460579
TTCGCGG1246600.031.6254448
CGCGATT761800.022.83570120-21
GCGATTT827350.020.9376420-21
CGATTTT844400.020.64678422-23
GTTCGCG29800.016.8631867
CTCGCGA1235850.016.55836118-19
TCGCGAT1220500.016.52772718-19
GCTCGCG1299050.015.85878316-17
CGCTCGC1330750.015.75176116-17
CCGCTCG1315150.015.72965714-15
CGCGGCC1362550.015.39110910-11
GCCGCTC1399200.015.09141514-15
Produced by FastQC (version 0.11.5)