FastQCFastQC Report
Wed 27 Apr 2016
SRR616849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63382650
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA809950.12778733612431795No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT673980.10633509327868115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT409250.025.0575892
TATAGAA445700.023.11861
CGCGATT400100.022.64470720
GAATTCG459200.022.405345
AATTCGC482850.021.1308676
TAGAATT491800.021.0483133
GCGATTT433650.020.88238721
CGATTTT438650.020.60845422
AGAATTC514250.020.0376054
ATTCGCG539150.019.1079147
TTCGCGG604850.017.1476978
TCGCGGC611000.016.9383439
CTCGTAT76400.016.90640642
AATCTCG80300.016.39399139
ACACGTC92400.016.21658113
TCGCGAT632900.016.04293619
CTCGCGA636850.016.02821718
GCTCGCG673800.015.43307817
TCTCGTA83000.015.42681241
CCGCTCG684400.015.21715715
Produced by FastQC (version 0.11.5)