FastQCFastQC Report
Wed 27 Apr 2016
SRR616848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66229533
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1112430.1679658529375407TruSeq Adapter, Index 2 (100% over 51bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1017010.15355838308568476No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT885300.13367148459283262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG163600.033.576739
CTCGTAT179350.031.3552942
TCTCGTA181400.030.7655941
ACACGTC197000.030.27605613
CGTCTGA204400.029.36694316
CGATGTA197400.029.3287834
CACCGAT205000.028.91039831
ACGTCTG207750.028.83931515
ACCGATG208600.028.49775932
TCGTATG201350.028.40956743
CCGATGT204950.028.29176133
ATCTCGT198800.028.15223740
GTCACCG212050.027.82037429
CACGTCT215350.027.80070314
CGTATGC208750.027.68258344
CACACGT217300.027.37521212
TCACCGA216500.027.31189230
ATAGAAT469150.025.9729392
GCACACG237400.025.20906311
GTATGCC238900.024.28299345
Produced by FastQC (version 0.11.5)