FastQCFastQC Report
Wed 27 Apr 2016
SRR616847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53314109
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1549200.29057974128386915No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1269510.23811895646610168No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT686370.1287407804189319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT510050.026.7717972
TATAGAA546750.025.0785921
CGCGATT493800.024.85526720
GCGATTT514950.023.81256721
CGATTTT515550.023.8023122
TAGAATT607000.022.5884723
GAATTCG612450.022.3434015
AGAATTC637000.021.5281834
AATTCGC641950.021.2430366
ATTCGCG719850.019.1504677
TTCGCGG791150.017.5639748
TCGCGGC792750.017.4944849
TCGCGAT809800.016.7066219
GATTTTT748800.016.63732123
CTCGCGA820300.016.49286518
CGCGGCC851450.016.35445610
GCTCGCG848150.016.28020317
CCGCTCG862850.016.08630415
CGCTCGC867950.015.9839916
GGCCGCT895950.015.53722813
Produced by FastQC (version 0.11.5)