FastQCFastQC Report
Wed 27 Apr 2016
SRR616846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62588939
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1682980.26889415716090026No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1360630.2173914467538745No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT827970.13228695249171743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT566300.023.76299920
TATCTCG75850.023.3153739
ATAGAAT654450.023.2335032
GCGATTT591450.022.74112121
CGATTTT592350.022.71805422
CGCCAAT83050.022.62147133
TATAGAA691250.022.0713521
CTCGTAT85450.020.5906542
GAATTCG759450.020.0064725
TCTCGTA87700.019.95974741
TAGAATT767550.019.8598333
TCGTATG90650.019.80656243
AGAATTC783400.019.4580214
GTCACGC99450.018.95884329
AATTCGC803150.018.8814876
ACACGTC104450.018.3314213
AGTCACG105850.017.79133628
CGTATGC102850.017.71963344
ATTCGCG890150.017.1649887
ACGCCAA113000.016.92439332
Produced by FastQC (version 0.11.5)