FastQCFastQC Report
Wed 27 Apr 2016
SRR616845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51123187
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT11249002.2003714283305538No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT6260061.224505037215305No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1829050.3577730785836963No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT897390.1755348311911775No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT685140.13401746647758872No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTC533370.10433035014033848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4306050.037.5199431
GCGTTTT2037300.036.09428845
ATAGAAT4580050.035.228153
TATAGAA4577100.035.20692
TAGAATT4676250.034.498944
GAATTCG4632000.034.4622736
AGAATTC4687300.034.2070925
AATTCGC4650250.034.200327
CGCGATA2260650.034.11016521
TAATACG2095750.033.7502326
GCGATAA2191000.033.7065222
ATTCGCG4719250.033.7060178
CGATAAT2182400.033.58631523
TCGCGAT4682800.033.50193420
AATACGA2049250.033.4887327
ATACGAC2140350.033.36390328
TCGCGGC4756000.033.3330110
TTCGCGG4777350.033.2834859
CGCGATT2243150.033.2351521
TACGACT2184550.033.2106829
Produced by FastQC (version 0.11.5)