FastQCFastQC Report
Wed 27 Apr 2016
SRR616845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51123187
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1565910.30630132663677634No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1036550.20275535639043785No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT692510.13545908239249638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT441500.025.8986382
TATAGAA463600.024.7337131
CGCGATT414150.024.2951320
GCGATTT437000.023.0144821
CGATTTT439700.022.8936322
GAATTCG512650.022.2383695
TAGAATT527800.021.7279243
CTCGTAT67600.021.30139242
AATTCGC540000.021.0245386
TCGTATG71450.020.8148643
AGAATTC558600.020.5016984
AATCTCG72000.020.43711339
CGTATGC76450.019.57124344
ATTCGCG601000.019.0515797
TCTCGTA76900.018.90077241
ACACGTC86800.018.2226513
TTCGCGG656950.017.6002678
TCGCGGC659750.017.4812379
TCGCGAT672650.016.62094919
CGTCTGA97000.016.5616216
Produced by FastQC (version 0.11.5)