FastQCFastQC Report
Wed 27 Apr 2016
SRR616844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47872386
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC2281950.476673546206784TruSeq Adapter, Index 2 (100% over 51bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1196960.2500314064145455No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1120120.23398039947288193No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT884750.18481426850126084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG287000.039.59762239
CTCGTAT297900.039.37234542
TCTCGTA299150.038.4707241
ACACGTC339900.037.52594413
CCGATGT321250.037.20390733
ACCGATG335450.037.1716932
CACCGAT337250.036.99994331
TCGTATG322200.036.97550243
ACGTCTG347250.036.66682415
CGTATGC326200.036.63934744
CACGTCT348450.036.59870514
CGTCTGA347400.036.47612816
ATCTCGT315800.036.45666540
GTCACCG345700.036.134629
TCACCGA345900.035.99011630
CACACGT358600.035.57538212
GCACACG368750.034.72421611
GTATGCC344050.034.65995445
GATCGGA394650.033.066411
ATCGGAA398450.032.7794762
Produced by FastQC (version 0.11.5)