FastQCFastQC Report
Wed 27 Apr 2016
SRR616843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50290819
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTT10539582.095726458541071No Hit
GTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTT6067051.2063931589580994No Hit
TGTATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTT1939310.3856190928208984No Hit
TGTATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTT958720.19063519327454181No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT717190.14260853457168793No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA653510.12994618361653643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA4170750.036.6315231
GCGTTTT1923650.035.7319645
TATAGAA4433800.034.369872
ATAGAAT4443600.034.352963
TAGAATT4546600.033.5795024
GAATTCG4517150.033.4445426
CGCGATA2172700.033.33181821
AATTCGC4530150.033.2653167
AGAATTC4560900.033.2280245
TAATACG2020150.032.98207526
GCGATAA2108500.032.9425422
ATTCGCG4590650.032.8376968
CGATAAT2102100.032.79215223
AATACGA1978900.032.68780527
TCGCGAT4545150.032.666820
ATACGAC2070400.032.52403628
TCGCGGC4634900.032.444610
CGCGATT2169700.032.41740821
TTCGCGG4651900.032.4075629
TACGACT2115350.032.37097529
Produced by FastQC (version 0.11.5)