FastQCFastQC Report
Wed 27 Apr 2016
SRR616843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50290819
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1491630.2966008567090546No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1096770.21808553167527459No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT862890.17158002537202666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGAAT452100.025.2825832
CGCGATT419600.024.6551720
TATAGAA470250.024.429641
GCGATTT441000.023.45875221
CGATTTT440850.023.44130722
GAATTCG527200.021.7226185
TAGAATT536400.021.3752123
CTCGTAT66550.021.13021342
AATTCGC542550.020.9877726
CGTATGC71250.020.90483544
TCGTATG71950.020.6388343
AGAATTC569750.020.143774
AATCTCG73100.019.54509439
ACACGTC86700.019.20379813
ATTCGCG600850.019.1123627
TCTCGTA74850.018.48652341
TTCGCGG661750.017.5370928
CGTCTGA93450.017.40755816
TCGCGGC667400.017.3414339
TCGCGAT682150.016.58074219
Produced by FastQC (version 0.11.5)