FastQCFastQC Report
Wed 27 Apr 2016
SRR616842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52384200
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1507620.2878005200041234No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT974050.1859434715047667No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT837750.15992417561020308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG69700.024.92104339
CTCGTAT72750.024.4015542
CGCCAAT77300.024.1298333
CGCGATT399550.023.22313920
ATAGAAT452050.023.2201542
GTCACGC83550.022.2977929
TCTCGTA79200.022.2154441
GCGATTT420250.022.05783521
TATAGAA486050.021.7964361
CGATTTT427000.021.69862722
TCGTATG88050.020.54471443
GAATTCG509950.020.5381895
AGTCACG92750.020.47417828
ACACGTC98150.019.8060413
TAGAATT533150.019.7752743
AATTCGC531450.019.576076
CGTATGC94000.019.43574144
ACGCCAA101400.018.88266832
ATCTCGT92300.018.86742440
AGAATTC565050.018.7225674
Produced by FastQC (version 0.11.5)