FastQCFastQC Report
Wed 27 Apr 2016
SRR616841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR616841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54927341
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1533970.2792725757469308No Hit
TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTTTTT1056270.1923031373391987No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT923180.16807294567563355No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC578710.10535918714870977TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG113650.026.23121643
AATCTCG110850.026.00145339
CTCGTAT111300.025.95624242
TCTCGTA111950.025.38348441
ACACGTC126450.025.28410713
CGTATGC118950.025.10031944
CGTCTGA132150.024.12575716
CGCGATT436700.024.00918620
ACGTCTG136750.023.51133215
ATAGAAT509400.023.3488272
GCGATTT456600.022.94799221
CGATTTT464000.022.6499622
TATAGAA532500.022.4063221
ATCTCGT129850.021.88433640
CACGTCT148050.021.68644114
CACACGT153050.021.16905812
GCACACG156750.020.98498311
GAATTCG584500.020.2589655
TAGAATT598600.019.9208373
AATTCGC611650.019.2567126
Produced by FastQC (version 0.11.5)